| Education
- Ph.D. in Biological Sciences, University of Arkansas, 1999-2003
Dissertation: CpSRP, cpFtsY, and Albino3, Components of the Signal
Recognition Particle Protein Transport Pathway in Chloroplasts, Function
in LHCP Integration and Form a Complex at the Membrane (Some Assembly
Required).
G.P.A. 4.0/4.0
- B.S. in Horticultural Science, University of Arkansas, Fall
1999
Summa cum laude, G.P.A. 3.98/4.0
Research Experience
- Doctoral Graduate Assistant, University of Arkansas, 1999-2003.
Project focused on identifying membrane components
of a protein translocon in the thylakoid membrane. Advisor: Dr. Ralph
Henry
- Research Assistant, Molecular Biology Lab, University of
Arkansas, 1998-1999
Insertion of a gene into Arabidopsis for protein production.
- Research Assistant, Tissue Culture Lab, University of Arkansas,
1996-1997
Assist with tissue culture and development of a regeneration/transformation
system for cowpea (Vigna unguiculata).
- Laboratory Assistant, Soybean Genetics Lab, University of
Arkansas, 1995-1996
Running agarose gels and washing glassware.
Publications (Note: until 2004 publications were under the name M. Moore)
- Moore M, Goforth RL, Mori H, Henry R. (2003) Functional interaction
of chloroplast SRP/FtsY with the ALB3 translocase in thylakoids: substrate
not required. J Biol.Chem. 162(7):1245-54.
- Yuan, J, A Kight, R Goforth, M Moore, EC Peterson, J Sakon,
R Henry. (2002) ATP stimulates SRP/FtsY-supported protein integration
in chloroplasts. J Biol. Chem. 277(35):32400-4.
- Jiang, F, L Yi, M Moore, M Chen, T Rohl, KJ van Wijk, JW
de Gier, R Henry, R Dalbey. (2002) Chloroplast YidC homologue Albino3
can functionally complement the bacterial YidC depletion strain and
promote membrane insertion of both bacterial and chloroplast thylakoid
proteins. J Biol. Chem. 277(22):19281-8.
- Woolhead, CA, S Thompson, M Moore, C Tissier, A Mant, A
Rodger, R Henry, C Robinson. (2001) Distinct Albino3-dependent and
-independent pathways for thylakoid membrane protein insertion. J
Biol. Chem. 276(44):40841-6.
- Mant, A, CA Woolhead, M Moore, R Henry, C Robinson. (2001)
Insertion of PsaK into the thylakoid membrane in a 'horse-shoe' conformation
occurs in the absence of signal recognition particle, nucleoside triphosphates
or functional Albino3. J Biol. Chem. 276(39):36200-6.
- Moore, M, MS Harrison, EC Peterson, R Henry. (2000) Chloroplast
Oxa1p homolog, Albino3, functions in post-translational integration
of the light harvesting chlorophyll-binding protein into thylakoid
membranes. J Biol. Chem. 275(3):1529-32.
- DeLille, J, EC Peterson, T Johnson, M Moore, A Kight, R
Henry. (2000) A novel recognition element in LHCP enables post-translational
binding by a chloroplast signal recognition particle. Proc Natl Acad
Sci. 97: 1926-31.
Poster Presentations
- Moore, M, F Jiang, R Dalbey, and R Henry. (2002) “Chloroplast
SecE requires ALB3 for integration into the E. coli inner membrane,
but not thylakoid membranes.” American Society for Biochemistry
and Molecular Biology, Experimental Biology; New Orleans, LA.
- Moore, M, and R Henry. (2000) “Composition of the
ALB3 translocon in chloroplast thylakoid membranes.” The American
Society for Cell Biology, Annual Meeting; San Francisco, CA.
- Moore, M, and R Henry. (2000) “Thylakoid localization
of chloroplast ALB3, homolog of mitochondrial Oxa1p.” American
Society for Microbiology, Macromolecular Transport Across Cellular
Membranes; Savannah, GA.
Summary of Acquired Skills
During my Ph.D. training, I have had the opportunity to use biochemical
assays to investigate protein transport in chloroplasts. Specifically,
I have identified the function of the ALB3 protein in thylakoids as
a requirement for the integration of the integral membrane protein,
LHCP (light-harvesting chlorophyll a/b-binding protein). This work has
made me proficient in DNA cloning, RT-PCR, protein expression and purification
(including extensive work with FPLC systems), Western blotting, and
other techniques involved in membrane protein studies (e.g. Blue Native
PAGE, co- and immuno-precipitation, crosslinking, and density gradients).
I am interested in continuing to use and improve my protein chemistry
skills to study protein function and/or interactions.
Teaching Experience
- Teaching Assistant, Biochemical Genetics, University of
Arkansas, Spring 2001
- Teaching Assistant, Introduction to Plant Biology, University
of Arkansas, Spring 1998
Honors and Awards
- NSF EPSCoR Fellowship Recipient, $15,000 per year, 1999-2002
- Doctoral Fellowship Recipient, University of Arkansas, $3,000
per year, 1999-2003
- American Society for Horticultural Science, Southern Region
Meeting, 1998
- 1st place in the Edmund Undergraduate Paper Competition
- Presidential Scholar for the College of Agricultural, Food
and Life Sciences, 1998
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